What is indirect ELISA? What are its advantages and disadvantages?
The indirect ELISA utilizes an unlabeled primary antibody in conjunction with a labeled secondary antibody. Since the labeled secondary antibody is directed against all antibodies of a given species (e.g. anti-mouse), it can be used with a wide variety of primary antibodies (e.g. all mouse monoclonal antibodies). The use of secondary antibody also provides an additional step for signal amplification, increasing the overall sensitivity of the assay.
