What is included in the QPCR mastermix?
Return to Reaction Conditions Q: What is the concentration of the samples used in the reaction ? A: Most reactions are done at 10 ng/ul (based on the starting total RNA amount in the cDNA synthesis). However, this can vary depending on level of target expression and amount of sample provided. The concentration will always be included on the provided setup sheet. Return to Reaction Conditions Q: What is the concentration of the primers and probes? A: This will vary depending on what detection system you use. The classic TaqMan primers are usually at 900 nM and the probe at 200 nM. However, this can be re-optimized if needed. The concentrations will always be included on the provided setup sheet.The Amplifluor concentrations will always be the same: 5’ primer at 10 nM, 3’ primer at 100 nM, and the Amplifluor Uniprimer at 100 nM.
