What is immunofixation electrophoresis?
Immunofixation electrophoresis (IFE) is a two-stage process combining agarose gel electrophoresis, (UPE or SPE), with immuno-precipitation. Proteins are separated electrophoretically on several tracks on a gel. Antisera specific to individual classes of molecules are added to each track. If specific classes of light chain are present, insoluble complexes form with the antisera, which can then be stained and detected.
