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What is immunoaffinity purification?

April 26, 2017immunoaffinity purification

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What is immunoaffinity purification?

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The peptide/protein is bound covalently to CNBr activated Sepharose 4B or SL gel in a column. The antibodies within the polyclonal pool that are specific to this antigen are allowed to bind. The unbound antibodies and other serum proteins pass through the column, and the antigen bound antibodies are eluted. The resulting purified antibody is highly specific. ProSci will provide you with the serum, flow through, purified antibody (a yield from 0.1 mg to 0.5 mg per ml of serum), re-usable column to the antigen of interest and ELISA results.