What is HPTLC (High Pressure Thin Layer Chromatography)?
Features of HPTLC 1. Simultaneous processing of sample and standard – better analytical precision and accuracy less need for Internal Standard 2. Several analysts work simultaneously 3. Lower analysis time and less cost per analysis 4. Low maintenance cost 5. Simple sample preparation – handle samples of divergent nature 6. No prior treatment for solvents like filtration and degassing 7. Low mobile phase consumption per sample 8. No interference from previous analysis – fresh stationary and mobile phases for each analysis – no contamination 9. Visual detection possible – open system 10. Non UV absorbing compounds detected by post-chromatographic derivatization Steps involved in HPTLC 1. Selection of chromatographic layer 2. Sample and standard preparation 3. Layer pre-washing 4. Layer pre-conditioning 5. Application of sample and standard 6. Chromatographic development 7. Detection of spots 8. Scanning 9. Documentation of chromatic plate Selection of chromatographic layer ยท Precoated p
