What is Horseradish Peroxidase (HRP)?
HRP is an enzyme-label that is commonly used in conjunction with a secondary antibody to detect proteins. It is extracted from the roots of horseradish using mild affinity chromatography. It has high levels of both activity and specifity and is therefore a preferred enzymatic label in procedures such as ELISA, Western Blots (immunoblotting), and immunohistochemistry (Horseradish Peroxidase 2000). What is an Enzyme-Linked Immunosorbent Assay (ELISA)? The ELISA is a method to determine the concentrations (on the order of ng/mL to pg/mL) of a material in a solution. The ELISA will measure optical density and using that information, you can determine the concentration of the protein of interest. In order to do this, you can create a standard curve graph for the optical density versus the concentration of the protein; extrapolation from this curve will yield the concentration of the protein. For more information on this tool, click here. How is HRP used in an ELISA? HRPs are used as seconda
