What is GreenScreen HC?
GreenScreen HC is a novel human cell-based screening assay for genotoxicity. The GreenScreen HC assay uses TK6 host cells which are p53 competent and familiar to most genetic toxicology laboratories. A patented GFP reporter system exploits the proper regulation of the DNA damage response gene GADD45a. The assay delivers both high specificity and high sensitivity and detects all common mechanistic classes of genotoxin. The 96-well microplate format takes about 20 minutes to prepare and delivers results after 48 hour incubation. A single microplate is sufficient for the simultaneous testing of 4 compounds over 9 serial dilutions. The protocol is also readily automated using standard laboratory equipment. For more information see GreenScreen HC. Gentronix’s latest release is GreenScreen HC S9, which incorporates exogenous metabolic activation using S9 fraction to allow the detection of pro-genotoxic compounds. The 96 well microplate layout is similar to the GreenScreen HC assay with the a
