What is Dual Polarisation Interferometry (DPI) and What Can it Be Used For?
Dual Polarisation Interferometry (DPI) is an important enabling technology for the rapid and sensitive monitoring of interactions between proteins and small molecules and the differentiation between specific and non-specific binding. DPI provides density and dimensional measurements, showing mass capture events and revealing conformational changes in proteins that are indicative of a response to specific binding. The sub-atomic resolution of DPI allows the detection of small molecules binding to large, immobilized proteins. This means that DPI can be used to determine whether small molecules change the conformation or structure of a protein upon binding, opening new insights into structurally informed drug discovery, giving a level of information beyond that provided by kinetic parameters alone.
