What is composition of conjugates?
We offer to our customers the conjugates based on polyacrylamide loaded with 20% mol. of oligosaccharide, in accordance with our experience this value is optimal. Carbohydrate probes additionally contain the biotin (5 %mol.), fluorescein (1 %mol.), or other label. The knowledge of the optimal conditions for the condensation reaction gives us the possibility to synthesize conjugates with the exactly defined molar content of ligands. The real size of the conjugate was evaluated by electron microscopy: the molecule had an almost perfectly spherical form with the diameter about 150Å, which agrees well with its hydrodynamic size by indirect methods. For the majority of bioanalytical problems, the molecular mass of conjugates of about 30-50 kDa is sufficient and probably optimal. More information about composition, properties, labels see in Review “Polyacrylamide-Based Glycoconjugates as Tools in Glycobiology” [Glycoconjugate Journal, 15, 431-446, 1998].
