What is autoinduction?
Autoinduction refers to bacterial cultures grown in media containing specific components that, after an initial period of tightly-regulated, uninduced growth, automatically induce target protein expression, without IPTG. Typically,autoinduced expression produces a greater proportion of soluble target protein than does IPTG-induced expression. Overnight Express™ Autoinduction Systems use optimized media components to promote culture growth to high cell densities, followed by lactose-induced protein expression from lac-based promoters. This method provides higher protein yields and greater convenience compared to standard IPTG induction.
