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What is Aqueous Normal Phase (ANP)?

April 26, 2017anp aqueous normal phase

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What is Aqueous Normal Phase (ANP)?

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[top] Aqueous Normal Phase (ANP) is a full, new mode of HPLC. Reverse Phase (RP) and Normal Phase (NP) HPLC are more well known but ANP offers chromatographers benefits that are not possible with other modes. In Aqueous-Normal Phase, the maximum retention time of target compounds is with 100% acetonitrile (least polar solvent) and as you increase the polar solvent content (Aqueous), the retention reduces to a minimum when the mobile phase is at 70% Acetonitrile. You get Normal Phase mode using Aqueous or Reverse Phase Solvents. Polar compounds that would not retain in RP for example, retain very well in ANP. By definition: Normal-Phase Chromatography (NP)is a chromatographic mode that will increase the retention of a target compound as the mobile phase becomes less polar (a decrease in concentration of the most polar solvent, often water) conversely the compound has longest retention in a non-polar solvent such as 100% hexane. The main separation mechanism is based on the functionality