What is a vector-based siRNA?
Vector-based siRNA is a novel and resilient means of harnessing the effects of siRNA and RNAi without costly and cumbersome traditional siRNA synthesis. In DNA vector-based siRNA technology, a small DNA insert encoding a short hairpin RNA sequence that targets the gene of interest is cloned into a commercially available siRNA vector. The insert-containing vector can be transfected into the cell, where it expresses the short hairpin RNA. The hairpin RNA is rapidly processed by the cellular machinery into a 19- to 22-nucleotide double-stranded RNA (siRNA). • What are the advantages of vector-based siRNA? Vector-based siRNA is more stable and easier to handle than traditional siRNA. Since it is absorbed into the host cell’s DNA sequence, it can be used to establish stable cell lines and knockout mouse lines. Please click here to learn more. • What is the difference between vector-based siRNA and siRNA cassettes? Vector-based siRNA allows for the establishment of stable cell lines and each
