What is a validation test?
In order to use the comparative CT method of calculation, a validation test must be run. The validation will determine if the efficiencies of the target and the endogenous control amplifications are approximately equal. A validation test requires a serial dilution of a sample that expresses the target(s) of interest to be run along with the endogenous control. The endogenous control CT will be subtracted from the CT of the target at each dilution. The delta CT’s will be plotted against the log input amount of the serial dilution. If the slope is less then 0.25, the Comparative CT method can be used. If the slope is greater than this, the standard curve method must be used.
Related Questions
- What brand of HPLC/GC column was used in the development and/or validation of a particular test? Is there an alternative chromatographic column for a particular test?
- Is it necessary to test the growth promotion on all received batches or does it serve just for microbiological validation?
- Does QEngine support validation of the response content received during load test ?
