What is a synthesis scale?
The synthesis scale is based on the amount of the first base attached to the solid support (controlled-pore glass, CPG) to start the oligo synthesis, not the amount of the final material synthesized. For larger scales, the amount of solid support is increased. The scale does not indicate the expected yield. Losses in yield may occur during synthesis, post-synthetic processing, transfer of material and quality control. The quantity of DNA ultimately received is usually lower than the theoretical yield. For example, a 20 mer synthesized at a 200 nmole scale will produce approximately 80 nmoles.
