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What is a subtraction library?

April 26, 2017library subtraction

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What is a subtraction library?

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A subtraction library is a collection of genes that are differentially expressed between two different populations. Suppression subtractive hybridization (SSH) is one of the most powerful and popular PCR-based methods for isolating differentially expressed transcripts. Using a small quantity of either total RNA or poly A+ mRNA (2.5 ug) from each of two populations, the SSH procedure simultaneously subtracts and partially normalizes the abundance of target cDNAs in the subtracted population. Two subtraction libraries, forward (“experimental”) and reverse (“control”), are generated by each subtraction protocol.