What is a Standard Assay?
The establishment of standard curves is a concept based on a direct relationship between two parameters such that if you can determine for certain one part of the related association, you can derive the other. This requires that you have one parameter that is precise, definable, and measurable. This utility is extended to the biological and chemical laboratory as a tool used to determine an unknown concentration of substance in a particular volume (suspension) of liquid. By measuring the fixed and known concentrations of a substance, you may derive another physical component of that suspension whether it be radiological, fluorescent, luminescent, or other. In a more focused application, the optical density is the derived physical descriptor that is dependent on the concentration of protein in suspension. Knowing the value of one component, called the independent variable, and plotting it against the unknown yet related component, called the dependent variable, produces a two-dimensiona
Related Questions
- How comparable are the results obtained with the FLIPR Calcium Assay Kit to those from standard protocols using the conventional fluo-3 and fluo-4 dyes?
- Can you provide the general basis of the assay kits and how to calculate concentrations from the standard curve and sample data?
- Where can I find the Standard Operating Procedures for the in vitro lead bioaccessibility (IVBA) assay?
