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What is a sandwich ELISA?

April 26, 2017ELISA sandwich

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What is a sandwich ELISA?

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A sandwich ELISA uses an immobilized capture antibody specific for the analyte of interest in a sample. The analyte of interest is then bound to the immobilized antibody. A labeled secondary antibody, specific for the analyte of interest, is used for detection. The analyte of interest is “sandwiched” between the two antibodies. Values are quantitative when compared with a standard curve.