What is a possible alterternative oxidizing solution to I2 ?
RESPONSE:In situations where you want to avoid the use of iodine containing oxidizing solutions, such as when using 7-deaza-dG, or when you want to use a non aqueous oxidizing solution t-butyl hydroperoxide (TBHP) has been shown to work both for DNA (1) and RNA (2). Reagents: TBHP, anhydrous, 5-6M solution in decane (Aldrich 41,666-5) Methylene chloride For DNA small scale: 1.1 M TBHP in Methylene Chloride, 0.8 min oxidation (1) For large scale RNA synthesis: 37ml TBHP solution + 63ml Methylene Chloride (approx. 2M), 6 min oxidation (2) Mix reagents fresh prior to use, oxidizing solution only stable for a few days on synthesizer. REFERENCE(S): 1. Hayakawa, Y., et al., JACS, 1990, 112, 1691. 2. Sproat, B., et al., Nucleosides & Nucleotides, 1995, 14, 255.
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