What is a Full ORF Shuttle Clone? How is it related to Gateway® Expression Clones?
The Gateway Technology is a universal cloning method that takes advantage of a site-specific recombination reaction (“shuttle”) using att sites in the vector to provide a rapid and highly efficient way to move your gene of interest into various expression vectors. First, an Entry Clone containing the gene of interest flanked by attL sequences is generated. imaGenes can offer different clone collections of such Entry Clones (i.e. “Full ORF Shuttle Clones”). Once a gene is cloned into an Entry vector you can transfer the ORF insert into one or more expression vectors by a highly efficient, site-specific recombination with the attR sequences to create your desired Destination Clone. Thereby, the Gateway Technology circumvents traditional restriction enzyme-based cloning limitations – orientation and reading frame are maintained by the recombination reaction, and there is no need for further sequence validation. These ready-to-use Expression Clones are also available at imaGenes.
Related Questions
- I am interested in some clones from NIA 15k and NIA 7.4k Mouse cDNA Clone Sets. How can I obtain individual clones of NIA 15k and NIA 7.4k Clone Sets?
- Do all of your clones exhibit reporter gene (lacZ, gfp, venus, depending on vector) expression?
- What is a Full ORF Shuttle Clone? How is it related to Gateway® Expression Clones?
