what is a fibroblast conditioned medium?
A cell lysate is what you get when you break cells. It is really hard to break most plant, and bacterial cells because they have cell walls, but to breal animal cells all you have to do is to add water to them. Because of the lack of cell walls, animal cells will just explode and release their contents. This is why when we use eye drops or nose drops or IV, the salt concentrations is similar to that in our cells, “saline solution”. Fibroblast conditioned medium is a waste product of growing animal cells in a Petri dish. The cells stick to the bottom of the dish (this is what normal cells will do) and every day or so you remove the old medium (now “conditioned”) and add fresh medium with all the nutrients and other stuff those cells need to stay alive and reproduce. If the cells are fibroblasts, a type of cell, then you get fibroblast conditioned medium.
