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What is a Direct/Indirect ELISA?

April 26, 2017ELISA indirect

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What is a Direct/Indirect ELISA?

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In a direct ELISA, a plate is coated with the antigen of interest and a detection antibody is used to verify its presence. This technique is only considered semi-quantitative when compared with the recombinant standard. Please refer to the Indirect ELISA Protocol.