What is a difference between MeDIP kit vs MagMeDIP kit (DNA purification vs no DNA purification)?
The protocol proposed in MagMeDIP kit is faster as due to magnetic beads wash steps are shorter. Secondly, the protocol is more flexible concerning DNA purification after IP. The composition of DNA isolation buffer is different from elution buffer in MeDIP kit. It allows the direct use of isolated DNA in qPCR without additional purification. Anyway, for other sensitive applications like amplification, DNA should be purified. You need to perform the complete protocol: genDNA isolation, fragmentation, immunoprecipitation, elution and purification. It is recommended to analyse MeDIP by qPCR before starting microarray study.
