What introduced genetic constituents do the strains contain?
The strains we are distributing are modified in that they contain a derivative of the pTiBo542 plasmid from Agrobacterium tumefaciens. pTiBo542 had been “disarmed” by having both of its T-DNA regions deleted, to create pTiEHA101 (with an nptI gene in place of the deleted T-DNA) and pTiEHA105 (with no foreign DNA inserted in place of the deleted T-DNA). For details, see Hood et al 1986 J Bact 168:1291-1301 and Hood et al 1993 Trans Res 2:208-218. pTiEHA105 was modified at CAMBIA by homologous recombination insertion of a pCR2.1TOPO plasmid (Invitrogen Inc.) containing an RK2 oriT (amplified from pSUP202 with primers EVS49 and EVS50 – see Stabb and Ruby 2002 Methods Enzymol. 358:413-426) and the virG gene – to create pTiWB1 – or the moaA gene – to create pTiWB3. Some strains also contain the binary vector pCAMBIA1105.1R with a GUSPlus reporter construct (see the GUSPlus Project).
