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What if I want to clone or sequence the PCR product? Can I use the kit with Phusion?

April 26, 2017clone kit PCR Product sequence

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What if I want to clone or sequence the PCR product? Can I use the kit with Phusion?

1 Answer

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Yes. Due to the extremely high inhibitor resistance of Phire and Phusion both DNA polymerases can be used with plant material. However, it is good to keep in mind that kit is optimized for Phire. Therefore the results may be less optimal with Phusion.