What if I want resequencing of a nonstandard gene isoform?
Coverage estimates for all reference gene isoforms are available using a precomputed estimates table. An investigator may submit a FASTA file of the cDNA or a set of genomic coordinates on the current NCBI human genome sequence of the region to be targeted if it is nonstandard. This should be noted in the RS&G application and discussed further with the Resequencing Center upon project approval. Following these discussions with the Resequencing Center, the targeted gene isoform cannot be altered.
