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What if I put excess protein through the PhyTip column, does that affect my ability to capture the protein I want?

April 26, 2017ability affect capture column excess phytip protein

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What if I put excess protein through the PhyTip column, does that affect my ability to capture the protein I want?

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No, once all capture sites are full, excess protein does not affect the existing captured proteins. This process will produce an extremely concentrated purified protein when the sample is finally recovered in the small elution volume.