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What if I only have picogram amounts of RNA?

April 26, 2017amounts RNA

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What if I only have picogram amounts of RNA?

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We have amplified as little as 500 pg of total RNA using a modified technique we call double SPIA. The facility staff has been successful in generating enough cDNA for further processing from as low as 60pg/ul RNA input when using 2.5X SPIA reagents.