Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What histone control sample should be used for ChIP?

April 26, 2017chip CONTROL histone sample Used

0

0 Posted

What histone control sample should be used for ChIP?

1 Answer

0

0 Posted

Calf thymus histone preparation should be used as a positive control histone sample for checking antibody specificity in western blot. When chipping for histone modifications, purified histone H3 and H1 can be used as positive controls for the quality of the experimental histone preparation (histone H1 is usually used for X-ChIP).