What hardware is needed for the read-out of the PepStar microarrays ?
– minimum resolution: 50 um / Pixel – focusing should be possible – intensity should be adjustable – size of slide: 1 x 3 inch – size of scanned area should be at least 63 x 21mm – extinction/emision filters depending on the fluophores used # For High Density-Peptide Microarrays (more than 3 x 384 Peptides/Microarray): – minimum resolution: 10 um / Pixel – focusing should be possible – intensity should be adjustable – size of slide: 1 x 3 inch – size of scanned area should be at least 63 x 21mm – extinction/emision filters depending on the fluophores used – software that is capable of reading and analyzing .gal-files. JPT is working with a Genepix Axon 4000B Scanner with two lasers and with a Genepix 4200 with three lasers. The picture should be at least a 8-bit tiff-Image, because then it will be compatible with most softwares.
