What hardware is needed for read-out of the Enzyme Substrate Sets?
For radioisotobic read-out of the kinase substrate set, a Streptavidin membrane (Promega, SAM-membrane) is needed, as well as a system for detecting incorporated radioisotopic labeled Phosphate (Phospho-Imager). For the Phosphatase Substrate Sets, a microtiterplate-reader capable of detecting absorbance at 620-660nm is needed. For the Protease Substrate Sets, a microtiterplate-reader capable of detecting fluorescence (Ex: 350nm, Em: 490nm). Preferred read-out procedure is from top but microtiterplates with clear bottom are also available.
