What happens to the U/g Hb values when screening for G-6-PD at different temperatures?
As a general rule of thumb, the speed of the reaction is doubled if the temperature is risen by 10 degrees. This is also valid for the enzyme activity. The lower the temperature the less activity is observed. Moreover, at low temperatures (less than 18 degrees) the enzyme may not work at all or shift from the rule (the activity will be far less than the theoretically calculated). For temperatures over the 22 mark, one can estimate (roughly) that the activity will rise by 10% for every degree Celsius. Thus, a value of 10 U/g Hb at 22 degrees will become 18 U/g Hb at 30 degrees. Similarly a value of 10 U/g Hb at 30 degrees will become 17 U/g Hb at 37 degrees. If controls of known activity are used for the evaluation of the samples then the experiment can be run at 30 degrees and the results expressed at 37 degrees by multiplying the results by the control values’ ratio. This is a rough estimation but it makes a lot of sense if you perform a confirmation analysis in an analyzer which will
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