What happens if there are no cysteines or methionines in my sample to use as an internal standard?
Only one such sample has been presented so far! As the number of sulphurs is used to get the number of atoms of the element of interest (X) from the ratio of the sulphur/X concentrations measured, it is not possible to obtain accurate quantitation if there is no sulphur in the sample. Absolute concentration measurements are very difficult, and we have not attempted them. For the sample we have already analysed which had no sulphur, we were able to identify the metal present but not give a stoichiometric ratio for it with the protein.
Related Questions
- Can you provide the general basis of the assay kits and how to calculate concentrations from the standard curve and sample data?
- Why was the standard sample taken in the bedroom and living room, and not the basement or the attic?
- What happens if there are no cysteines or methionines in my sample to use as an internal standard?
