What happens if the media in the spin column dries up?
The agarose affinity support is in the form of hydrogel beads and always needs to be hydrated or soaked in PBS buffer. In addition, the affinity ligands are proteins that need to be in the native conformation to be active in binding. If the column bed appears to be still intact, the column may be soaked overnight in Equilibration Buffer and then equilibrated using the prescribed procedure before use. However, if the column bead has collapsed or dried into a thin film, it should be discarded because there is no guarantee that full activity can be reconstituted after re-equilibration.
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