What happens if a single protein in the mixture makes up a significant percentage of the total protein content, e.g., albumin in serum?
As with traditional 2-D methods, proteins of low abundance may be harder to visualize, and the highly abundant protein will probably obscure spots of lower abundance. The single protein will effectively take up most of the available CyDye™ DIGE Fluor minimal dye during labelling. To reduce this titration effect, pre-fractionation of the sample can be carried out to remove the interfering protein.
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