What guidelines exist for choosing a housekeeping gene for normalizing qPCR results?
If you are unsure of the correct housekeeping gene(s), review the literature in your field to determine which gene(s) other researchers like yourself commonly use. It is recommended that multiple housekeeping genes be utilized for each gene expression experiment, in order to account for any impact that an experimental condition may have on the expression of an individual housekeeping gene. For a systematic assessment of which housekeeping genes are appropriate for your specific experimental conditions, we recommend using the SABiosciences Housekeeping Genes PCR Arrays for human (PAHS-000), mouse (PAMM-000), or rat (PARN-000). These arrays consist of 8 sets of 12 common housekeeping genes. They are a valuable tool for easily identifying genes with a constant level of expression among your different experimental conditions.
