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What filter criteria does iRNAchek employ at design of siRNAs?

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What filter criteria does iRNAchek employ at design of siRNAs?

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A. iRNAchek lists all siRNAs that meet selected pattern criteria, but avoid selected undesirable signal, and finally falls within the given GC% range. Further, iRNAchek allows user to filter the list by ORFs, or eliminate by motifs, secondary structures, or any annotations. As a final step, one may also filter siRNAs based on BLAST hits.

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