What else do I need to complete an experiment using the SureSilencing™ shRNA plasmids?
To perform a complete and successful experiment with the SureSilencing™ shRNA plasmids, you will also need the following reagents: competent E. coli cells (with LB, agar, and ampicillin), a transfection reagent (we recommend using SureFECT), neomycin (if performing a stable transfection with neomycin plasmids), puromycin (if performing a stable transfection with puromycin plasmids). If you intend to utilize shRNA plasmids carrying the GFP expression cassette, you will need to have access to a FACS core facility. You will also need reliable qRT-PCR reagents for definitive validation of gene expression knock down. SABiosciences offers a complete line of exceptional qRT-PCR reagents. These include the RT2 qPCR-Grade RNA Isolation Kit (PA-001); RT2 qPCR First Strand Kit (C-03); complementary RT2 qPCR Primer Assays and SYBR Green-based RT2 qPCR Master Mixes; and RT2 Profiler™ PCR Arrays.
Related Questions
- How can I use the SureSilencing™ shRNA plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
- What else do I need to complete an experiment using the SureSilencing™ shRNA plasmids?
- How do I download the Users Manual for the SureSilencing™ shRNA plasmids?
