What downstream applications is the extracted RNA suitable for?
The cytoplasmic lysate can be used in 1- and 2-step RT-PCR, 1- and 2-step QRT-PCR, miRNA detection by QRT-PCR and RNA amplification reactions where limiting amounts of RNA are amplified using a T7 or T3 RNA polymerase system. For applications such as northern blotting that require more concentrated samples the RNA from the lysate would need to be concentrated or purified.
