What does the Wrangle button along the ImaGene toolbar do?
A. The wrangle feature of ImaGene applies new, stricter constraints to the results of spot localization without requiring further spot finding. Essentially, this allows users to reduce the spot search radius without redoing the spot finding. The benefit of this feature is to assist processing for those with either slower computer hardware or for those with numerous spots. A sample application would be to perform spot finding for a grid geometry on an array image with a local flexibility set a large number of pixels. After spot finding, if the resulting circle placement has high variability, the Local Flexibly can be reduced. After reducing Local Flexibility, click the Wrangle Button to apply the new setting without waiting for spot finding to be performed again. Most users with recent computer hardware need not employ this feature as it has become largely unnecessary.
