What does the purity mean on the HPLC report?
The purity of the peptide is determined by HPLC analysis with detection at 220 nm. The purity of the peptide does not take into account water and salts that are usually present in the sample. Other contaminates found with the peptide can range from sequence deletion, truncation or incompletely deprotected sequences. The peptide’s purity is the amount of correct peptide relative to all analytes that absorb at 220 nm.
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