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What does the M30-Apoptosense assay contribute that the Caspase-3 assay, or other caspase assays, does not?

April 26, 2017assay assays caspase caspase-3 contribute m30-apoptosense

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What does the M30-Apoptosense assay contribute that the Caspase-3 assay, or other caspase assays, does not?

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The assays measure different processes. The Caspase-3 assay measures enzyme activity while the M30-Apoptosense assay measures the accumulation of a caspase cleaved product. Due to product accumulation over time, M30-Apoptosense is more sensitive and convenient for screening of many samples. Caspase-3 assays can only be used with samples from cell extracts, while the M30-Apoptosense assay can be used with culture supernatants and serum or plasma.