What does the DYEnamic™ ET terminator cycle sequencing kit offer me that I cannot achieve with BigDye™ terminator kit?
• Shorter cycling time (1 min vs. 4 min) • Excellent performance on difficult templates (GC-rich, tandem repeat, etc.) • Salt tolerance (100 mM compared to less than 50 mM) • Easy post sequencing clean-up (fewer dye blobs) Additionally, Amersham Biosciences has considerable expertise in the field of separation media. As an alternative to ethanol precipitation, sequencing samples can be purified using gel filtration columns. For example, the AutoSeq™ 96 Plate uses a combination of chromatographic separation media (Sephadex™ G-50), PVDF membrane, and centrifugation in a 96-well format to remove excess salts and unincorporated fluorescent ddNTPs. The sequencing chemistry and the post sequencing clean-up reagents are offered in a cost-effective bundling price.
Related Questions
- Im a new customer who has never used DYEnamic ET Terminator Kit on the ABI Prism instrument before. What are the products that Ill need to order for the first time set up before testing the kit?
- Can both DYEnamic ET Terminator and BigDye terminator sequencing products be run together on the same slab gel?
- Can the DYEnamic ET Terminator Cycle Sequencing Kit be used with ABI 3100?
