What does MLP bind to?
Take a mouse cardiomyocyte and extract the cellular protein. Separate the proteins by SDS-PAGE and probe with MLP. Take the proteins bound by MLP, find the amino acid sequence, and sequence the gene for that particular protein. This assay could then be done with MLP-bound proteins to find other proteins involved in the process. Or, one could perform the two-hybrid system developed by Chien and associates to not only find proteins that bind to MLP but also to sequence them. Yet another option would be to ligate an oligonucleotide sequence coding for MLP to agarose beads and running the cardiomyocyte cellular proteins through the bead column. Wash with increasing concentrations of salt solution and collect proteins that bind to MLP. These same experiments could be done using the LIM1 motif of MLP only or the LIM2 motif only in order to see which parts of MLP bind to what. Which genes affect the penetrance of MLP? What are some common motifs specific to adult phenotype mice which are not
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