What does Chimera Detection do?
Chimera Detection looks at the number of unique oligomers in common between the unknown sequence and the most similar database sequence. It generates a histogram to visually indicate a hypothetical break-point in your sequence that would split your unknown sequence into two fragments that are of chimeric origin. • Q: Is this possible to study possible chimeric sequences in our own database of sequences and exclude the RDP database? Our partial sequences are approximately 450 nucleotides long. A: It is possible to compare your sequences to each other and to the sequences in the RDP dataset at the same time, but the RDP dataset can’t be excluded. When pasting your sequences in, be sure to paste them in both boxes – the “sequence upload” and the “expand RDP” boxes. For ease of interpretation, have the results emailed to you. Using a test set of closely related sequences not found in the RDP dataset, we found that the top twenty matches for most of the sequences had, at most, two or three
