What do the Ph1, Ph2 and Ph3 designations on the phase contrast objectives mean?
Phase contrast objectives function with a matching annular ring aperture in the condenser assembly. The diameter of this aperture varies with the NA of the objective being matched. For Olympus UIS objectives, Ph1 signifies objective NA of up to 0.50; Ph2 signifies NA of between 0.55 and 1.0; and Ph3 signifies NA above 1.0 (oil immersion objectives). Long working distance objectives use special-purpose phase rings.