What do strain generation prefixes like “G”, “F”,”N”, and “NE” mean?
“G” is a generic prefix for “generation”, used predominantly in mutagenesis schemes (e.g. where G0 is the mutagenized generation, G1 is the first generation, etc). “F” is strictly for “filial” generation, i.e. progeny of matings between siblings. “N” stands for backcross matings, i.e. back to the parent or parental strain. “NE” stands for N-equivalent and is used when a subsequent generation is crossed back to a prior one.