What do I need to complete a RT² qRT-PCR Assay?
You need: 1. A SABiosciences RT² SYBR Green qPCR master mix that matches the qPCR instrument in your laboratory; 2. RT² qPCR Primer Assays for your target genes; 3. A Housekeeping gene RT² qPCR Primer Assay. We also recommend using our RT² First Strand cDNA Synthesis Kit (Cat# C-03) for reverse transcription.