What difficulties are caused by printing the arrays too close to the black PTFE well patterning?
The well edges in the PTFE patterning have a maximum deviation of 100 m. The printer software should be set up to print the arrays at least 550 m away from the theoretical well edge. If the pins are set to print any closer there is a risk of printing over the patterning. Printing too close to the well edges may also cause problems during the hybridization / incubation step. For example, if the silicone superstructure is not accurately placed, it is possible that some spots may be very close to, or even under the superstructure. In this case it will be impossible to hybridizing or scan these spots.
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- What difficulties are caused by printing the arrays too close to the black PTFE well patterning?
