What determines the tropism of Adenovirus in infecting cells?
A. Cell lines with a high number of Adenovirus receptors (CAR and Integrin), like Cos-7 and HeLa, typically show significant Adenovirus infection, while cell lines with low numbers of adenovirus receptors (e.g. NIH 3T3 and U-937) show little and need higher doses (MOI) of viruses to infect all the cells. Reference: Seth, P., M. Rosenfeld, et al. (1994). “Mechanism of enhancement of DNA expression consequent to cointernalization of a replication-deficient adenovirus and unmodified plasmid DNA.” J Virol 68(2): 933-40..
