What coverage can be requested?
Four types of coverage can be requested: (1) Protein coding exons only; (2) all exons (UTR and coding) only; (3) NHLBI standard coverage, which consists of exons, conserved noncoding sequences plus 2 kb upstream and 2 kb downstream of the gene(s) across a region(s); and (4) NHLBI full (complete) coverage of the gene(s) or across the region(s) including all coding and noncoding sequences, plus 2 kb upstream and 2 kb downstream of the gene(s) or region(s). In addition, only regions refractory to PCR amplification or sequencing (e.g., low-complexity repeats) are excluded. For these four options, precomputed estimates for the sequences have been identified and calculated to aid the investigator in defining the size of the overall project.
